hepg2 c3a cells  (ATCC)

Journal: bioRxiv

Article Title: PKA–CIP4 SIGNALING REGULATES CIP4 RELOCATION IN ACTIVATED NATURAL KILLER CELLS

doi: 10.64898/2026.04.22.720117

Figure Lengend Snippet: C3A cells stably expressing CIP4 T225A or CIP4 T225E as GFP fusion proteins were fixed, stained for α-tubulin, and analyzed by confocal microscopy. A) Images show merged staining of α-tubulin (red) and GFP fluorescence indicating the expression of each CIP4 mutant (green). Insets show higher-magnification views of the merged staining and the GFP channel (grayscale) from the boxed areas. The dot plot shows quantification from four independent experiments of the percentage of CIP4 fusion protein associated with microtubules for each mutant, assessed as shown in . At least eight cells were analyzed per experiment. Scale bars, 5 µm. B) Microtubule-enriched fractions and associated proteins were prepared, and levels of CIP4 proteins in each fraction were analyzed by inmunoblot. The image shows a representative immunoblot for CIP4, including bands corresponding to endogenous CIP4 (75 kDa) and the fusion protein (100 kDa, CIP4-GFP). The dot plot shows quantification of the percentage of CIP4T225A and CIP4T225E associated with microtubules from four independent experiments. Each dot in the dot plots represents an independent experiment, shown in a different color to indicate paired data. Data are presented as mean ± SEM.

Article Snippet: C3A cells (HepG2/C3A; RRID: CVCL_1098) were obtained from the American Type Culture Collection (ATCC) by Dr. Pablo J. Schwarzbaum (IQUIFIB, CONICET–Universidad Nacional de Buenos Aires) in 2005.

Techniques: Stable Transfection, Expressing, Staining, Confocal Microscopy, Fluorescence, Mutagenesis, Western Blot

Journal: Journal of Medicinal Chemistry

Article Title: Discovery of Sulfonamide Pantothenate Kinase Activators and Elucidation of the Role of Isoform Selectivity in Cellular Pantothenate Kinase Activation

doi: 10.1021/acs.jmedchem.5c03452

Figure Lengend Snippet: Binding site view of the PZ-3022 (1) PANK3 AMP-PNP complex (PDB ID: 6PE6 ) showing the spatial proximity of the cyclopropyl to the ATP-binding pocket of the enzyme. B) Pantazine PZ-3890 CoA elevation dose response in human C3A cells demonstrating a “hook effect” and an optimal activity window of 1–30 μM for this compound.

Article Snippet: Human C3A (HepG2/C3A) cells (ATCC CRL-10741) were used for cellular assays.

Techniques: Binding Assay, Activity Assay

Journal: Journal of Medicinal Chemistry

Article Title: Discovery of Sulfonamide Pantothenate Kinase Activators and Elucidation of the Role of Isoform Selectivity in Cellular Pantothenate Kinase Activation

doi: 10.1021/acs.jmedchem.5c03452

Figure Lengend Snippet: Correlation Analysis and Surface Plasmon Resonance. A. Correlation analysis of the difference between K i of PANK1β and PANK3 vs C3A CoA elevation. The green-to-red scale shows the difference in K i between PANK1β and PANK3, while the size of the circles indicates the level of CoA elevation. B–E Surface Plasmon Resonance trace showing the binding of 20 and 21 to PANK3 and PANK1β in the presence of 1 mM ATP. B. 20 with PANK3. C. 20 with PANK1β. D. 21 with PANK3. E. 21 with PANK1β. The green data line was fit to a kinetic model shown in the black line. The association ( K a ), dissociation ( K d ), binding equilibrium ( K D ) constants, residence time (RT), and response units (RU) are shown in each panel.

Article Snippet: Human C3A (HepG2/C3A) cells (ATCC CRL-10741) were used for cellular assays.

Techniques: SPR Assay, Binding Assay